Breaking Down the Barrier: Evidence against a Role for Heparan Sulfate in Glomerular Permselectivity
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چکیده
منابع مشابه
Breaking down the barrier: evidence against a role for heparan sulfate in glomerular permselectivity.
T he glomerular capillary wall is thought to function as both a sizeand charge-selective barrier. The concept of charge selectivity emerged from a series of now classic studies that used tracers such as dextran, peroxidase, and ferritin to evaluate the influence of molecular charge on glomerular filtration (1–5). The permeability of anionic derivatives of each tracer was lower than their neutra...
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Perlecan is a heparan sulfate proteoglycan and a major component of the glomerular basement membrane. To understand the role of heparan sulfate chains of perlecan in glomerular filtration, detailed analyses were performed of the kidneys of Hspg2(Delta)(3/)(Delta)(3) mice, whose perlecan lacks heparan sulfate attachment sites in N-terminal domain I. Macroscopic, histologic, and electron microsco...
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Native and de novo synthesized heparan sulfate-proteoglycan (HS-PG) of basement membranes from isolated whole glomeruli were characterized. Sepharose CL-6B chromatograms of [35S]sulfate-labeled de novo synthesized HS-PG extracted from whole glomeruli indicated identical molecular weight characteristics to that isolated from purified basement membranes (Mr of intact HS-PG approximately equal to ...
متن کاملEvidence for the existence of multiple heparan sulfate proteoglycans in the human glomerular basement membrane and mesangial matrix.
Heparan sulfate proteoglycans (HSPGs) are essential components of the glomerular basement membrane (GBM) carrying a strong anionic charge. A well-characterized extracellular HSPG is perlecan, ubiquitously expressed in basement membranes. A cDNA construct encoding domains I and II of human perlecan was expressed as a fusion protein with glutathione S-transferase. This fusion protein was used to ...
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ژورنال
عنوان ژورنال: Journal of the American Society of Nephrology
سال: 2007
ISSN: 1046-6673,1533-3450
DOI: 10.1681/asn.2007010086